图书简介

A New Toolbox to Analyze Enhancer-Promoter Functions.-Global Run-on Sequencing (GRO-Seq).-Illuminating Enhancer Transcription at Nucleotide Resolution with Native Elongating Transcript Sequencing (NET-Seq).-Low Quantity single strand CAGE (LQ-ssCAGE) Maps Regulatory Enhancers and Promoters.-Analyses of Promoter, Enhancer and Nucleosome Organisation in Mammalian Cells by MNase-Seq.-Measuring Chromatin Accessibility: ATAC-Seq.-High-resolution ChIP-MNase Mapping of Nucleosome Positions at Selected Genomic Loci And Alleles.-Sequential Chromatin Immunoprecipitation to Identify Heterotypic Nucleosomes.-Low Input Targeted Chromatin Capture (low-T2C).-Proximity Ligation-assisted ChIP-Seq (PLAC-Seq).-Analysis of Enhancer-promoter Interactions using CAGE and RADICL-Seq technologies.-Using Open Chromatin Enrichment and Network Hi-C (OCEAN-C) to identify open chromatin interactions.-Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C).-Native Chromatin Proteomics (N-ChroP) to Characterize Histone Post-Translational Modification (PTM) Combinatorics At Distinct Genomic Regions.-Using ChIP-SICAP to Identify Proteins That Co-Localize in Chromatin.-Genome-wide profiling of protein-DNA Interactions with Chromatin Endogenous Cleavage And High-Throughput Sequencing (ChEC-Seq).-Transcriptional Activation Of Heterochromatin by Recruitment of dCas9 Activators.-Deletion of Regulatory Elements with All-in-One CRISPR-Cas9 Vectors.-Simultaneous Tagmentation-based detection of ChIP/ATAC Signal with Bisulfite Sequencing.-Low Input Whole-Genome Bisulfite Sequencing.

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