Synthetic Metabolic Pathways:Methods and Protocols

图书简介

This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational cost-effective engineering of cell factories with improved performance. Chapters are divided into four sections focusing on the four steps of the iterative design-build-test-learn cycle related to modern cell factory engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Metabolic Pathways: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Parts characterization for tunable protein expression.- Enzyme nicotinamide cofactor specificity reversal guided by automated structural analysis and library design.- Bacterial genome editing strategy for control of transcription and protein stability.- An Automated Pipeline for Engineering Many-Enzyme Pathways: Computational Sequence Design, Pathway Expression-Flux Mapping, and Scalable Pathway Optimization.- Computational Approaches on Stoichiometric and Kinetic Modeling for Efficient Strain Design.- Extended Metabolic Space Modeling.- Computational Methods to Assess the Production.- Designing and Implementing Algorithmic DNA Assembly Pipelines for Multi-gene Systems.- An adaptive Laboratory Evolution Method to Accelerate Autotrophic Metabolism.- CRISPR-Cas9 toolkit for Actinomycete Genome Editing.- Assembly and Multiplex Genome Integration of Metabolic Pathways in Yeast using CasEMBLR.- A modified Gibson assembly method for cloning large DNA fragments with high GC contents.- Coupling Yeast Golden Gate and VEGAS for efficient assembly of the violacein pathway in Saccharomyces cerevisiae.- Multi-capillary Column Ion Mobility Spectrometry of Volatile Metabolites For Phenotyping Of Microorganisms.- Selection of Highly Expressed Gene Variants in Escherichia coli using Translationally-coupled Antibiotic Selection Markers.- Design, Engineering, and Characterization Of Prokaryotic Ligand-binding Transcriptional Activators as Biosensors in Yeast.- A Capture-SELEX Strategy for Multiplexed Selection of RNA Aptamers against Small Molecules.- High-throughput Microfluidics for the Screening Of Yeast Libraries.- Growth-Coupled Carotenoids Production Using Adaptive Laboratory Evolution.- Two scale 13C Metabolic Flux Analysis for Metabolic Engineering.

Trade Policy 买家须知

关于产品：
● 正版保障：本网站隶属于中国国际图书贸易集团公司，确保所有图书都是100%正版。
● 环保纸张：进口图书大多使用的都是环保轻型张，颜色偏黄，重量比较轻。
● 毛边版：即书翻页的地方，故意做成了参差不齐的样子，一般为精装版，更具收藏价值。
关于退换货：
由于预订产品的特殊性，采购订单正式发订后，买方不得无故取消全部或部分产品的订购。
由于进口图书的特殊性，发生以下情况的，请直接拒收货物，由快递返回：
● 外包装破损/发错货/少发货/图书外观破损/图书配件不全（例如：光盘等）
并请在工作日通过电话400-008-1110联系我们。
签收后，如发生以下情况，请在签收后的5个工作日内联系客服办理退换货：
● 缺页/错页/错印/脱线
关于发货时间：
一般情况下：
●【现货】下单后48小时内由北京（库房）发出快递。
●【预订】【预售】下单后国外发货，到货时间预计5-8周左右，店铺默认中通快递，如需顺丰快递邮费到付。
● 需要开具发票的客户，发货时间可能在上述基础上再延后1-2个工作日（紧急发票需求，请联系010-68433105/3213）；
● 如遇其他特殊原因，对发货时间有影响的，我们会第一时间在网站公告，敬请留意。
关于到货时间：
由于进口图书入境入库后，都是委托第三方快递发货，所以我们只能保证在规定时间内发出，但无法为您保证确切的到货时间。
● 主要城市一般2-4天
● 偏远地区一般4-7天
关于接听咨询电话的时间：
010-68433105/3213正常接听咨询电话的时间为：周一至周五上午8：30～下午5：00，周六、日及法定节假日休息，将无法接听来电，敬请谅解。
其它时间您也可以通过邮件联系我们：customer@readgo.cn,工作日会优先处理。
关于快递：
● 已付款订单：主要由中通、宅急送负责派送，订单进度查询请拨打010-68433105/3213。

Synthetic Metabolic Pathways:Methods and Protocols（Methods in Molecular Biology） 合成代谢途径：方法与方案 遗传学

Synthetic Metabolic Pathways:Methods and Protocols（Methods in Molecular Biology）

合成代谢途径：方法与方案
遗传学